1263 The application of melanocyte preparation and transplantation technology

Vitiligo is a common acquired disease of pigment loss. Though various non-invasive treatments (including local immunosuppression, ultraviolet light irradiation, etc.), transplantation cultured autologous melanocytes as cell suspension an emerging choice. An epidermal graft about 1cm2 was obtained by suction blistering from the abdomen patient, then dissociated into single cells and in different Melanocyte Culture Mediums (MelM, ScienCell Research Laboratories; melanocyte growth medium M2, PromoCell) at 37°C 5% CO2incubator to selectively expand melanocytes. We found that there are obvious differences media products. These include morphology, rate, markers melanin production. Further, we observed expression TYR, TRP1, c-kit others passages under each culture medium. Melanocytes MelM showed significantly lower content compared with those M2. Next, prepared recipient sites inducing sunburned vesicles nude mice methoxsalen controlled A (PUVA), followed suspended Progressive repigmentation for up 1 month, site without scarring end follow-up. This can be used basis production before clinical future improve effect pigmentation after treatment. Hence, engraftment effects may enhanced growing subjected conditions.